Split Fluorescent Proteins for Studying Protein-Protein Interactions
Many, many techniques are available to assess protein-protein interactions. One popular approach is to fuse a protein of interest to each part of a split fluorescent protein (FP) and measure the signal produced when the candidate proteins' interaction brings the pieces of the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence Complementation (BiFC). It can provide a qualitative or quantitative readout of the interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or even single-particle tracking of the bound complex.
Publisher URL: https://blog.addgene.org/split-fluorescent-proteins-for-studying-protein-protein-interactions
DOI: 7610.27895.e604e564-ba7b-4172-8da5-b8e323e133d1.1651664231
Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.
Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.