bioRxiv Genomics
bioRxiv Genomics
5 years ago

Ancestry informative alleles captured with reduced representation library sequencing in Theobroma cacao

Osorio-Guarin, O. E., Quackenbush, C. R., J. A., Cornejo
As the source of chocolate, cacao has become one of the most important crops in the world. The identification of molecular markers to understand the demographic history, genetic diversity and population structure plays a pivotal role in cacao breeding programs. Here, we report the use of a modified genotyping-by-sequencing (GBS) approach for large-scale single nucleotide polymorphism (SNP) discovery and allele ancestry mapping. We identified 12,357 bi-allelic SNPs after filtering, of which, 7,009 variants were ancestry informative. The GBS approach proved to be rapid, cost-effective, and highly informative for ancestry assignment in this species.

Publisher URL: http://biorxiv.org/cgi/content/short/407387v1

DOI: 10.1101/407387

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