Environmental Sustainability
Environmental Sustainability
4 years ago

Cold stress alleviation using individual and combined inoculation of ACC deaminase producing microbes in Ocimum sanctum

Suman Singh, Arpita Tripathi, Chandan Singh Chanotiya, Deepti Barnawal, Pooja Singh, Vikas Kumar Patel, Poornima Vajpayee, Alok Kalra
Cold stress in Ocimum sanctum is a major constraint to crop productivity in certain geographical regions. The current study was carried out to examine the effect of consortia of ACC deaminase producing microbes in reducing cold-induced damage in O. sanctum during winters to enable farmers to have O. sanctum as multi-cut crop. The effect of single and combined inoculation of ACC-deaminase producing four plant growth promoting bacteria (PGPB) including Brevibacterium halotolerans (Sd-6), Bacillus subtilis (Ldr-2), Achromobacter xylosoxidans (Fd-2) and Burkholderia cepacia (Art-7), and a biocontrol agent Trichoderma harzianum (Th) have been investigated. Inoculation of O. sanctum plant with Th+Fd-2 showed better photosynthetic efficiency, which could have resulted in enhanced fresh herb weight (83.78%), compared to un-inoculated control plants. Moreover, the inoculation of Th +Fd-2 improved the nutrient uptake, increased the accumulation of proline, starch, total phenolics, and reduced the accumulation of ACC (1-aminocyclopropane-1-carboxylic acid), a direct precursor for ethylene biosynthesis. Scanning electron microscopy showed the effective co-colonization of Th with Fd-2 on the root surface. Our results suggest that co-inoculation of Th+Fd-2 could be a green initiative that can improve plant growth and alleviate cold stress-induced damages in O. sanctum.

Publisher URL: http://link.springer.com/article/10.1007/s42398-020-00118-w

DOI: 10.1007/s42398-020-00118-w

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