Bioassay-guided isolation of antioxidant, antimicrobial, and antiviral constituents of Cordia dichotoma fruits
The fruits of Cordia dichotoma G. Forst. (Boraginaceae) are commonly used as medicinal material to treat various ailments. In this paper, we performed bioassay-guided fractionation and isolation of bioactive constituents from the 80 % ethanol ultrasonic extracts of C. dichotoma fruits. The preliminary bioassay revealed that the ethyl acetate fraction (EA) of the extract exhibited stronger antioxidant: 2,2′-diphenyl-1-picrylhydrazyl (ABTS) with half inhibitory concentration (IC50) = 6.71 ± 0.46 μg/mL, 2,2′-diphenyl-1-picrylhydrazyl (DPPH) with IC50 = 34.18 ± 0.52 μg/mL, Ferric reducing antioxidant power (FRAP) with the value of 134.35 ± 2.01 μmol Fe2+/100 g) and antimicrobial (Candida albicans, Escherichia coli, and Staphylococcus aureus with the inhibition zones of 10.0, 9.0, and 12.0 mm, respectively) activities than other fractions. The EA fraction was further fractionated by silica gel column chromatography, and 45 compounds including 40 phenolics were isolated by various column chromatography and identified by spectroscopic data analysis. Among them, 41 compounds were isolated for the first time from C. dichotoma, including three previously undescribed phenolic derivatives. Most of the compounds showed significant antioxidant activity. Part of the individual compounds showed moderate or weak antimicrobial activity. And some compounds showed moderate antiviral activity (with IC50 = 12.84–115.56 μg/mL) to influenza virus A/Hanfang/359/95(H3N2). Additionally, the structure-activity relationships of phenolic compounds were discussed systematically. Furthermore, the bioactivity contributions of individual compounds to fractions were discussed based on LC–MS and HPLC analysis. The results indicated that C. dichotoma fruits were a potential source of bioactive phenolic compounds, and the fruits extract could be applied as a preservative in food industry because of its potent antioxidant and antimicrobial activities.
Publisher URL: https://www.sciencedirect.com/science/article/pii/S0926669021007421
DOI: 10.1016/j.indcrop.2021.113977
Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.
Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.